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Image Search Results
Journal: Frontiers in Oncology
Article Title: Glutor, a Glucose Transporter Inhibitor, Exerts Antineoplastic Action on Tumor Cells of Thymic Origin: Implication of Modulated Metabolism, Survival, Oxidative Stress, Mitochondrial Membrane Potential, pH Homeostasis, and Chemosensitivity
doi: 10.3389/fonc.2022.925666
Figure Lengend Snippet: Glutor exerts cytotoxic action on DL cells. DL cells or thymocytes (1 × 10 5 ) harvested from the thymi of healthy mice without tumor transplantation were processed for immunoblotting for GLUT1 and GLUT3 as described in the Materials and Methods section (A) . DL cells (1 × 10 5 ) were incubated in a medium alone or containing the indicated concentrations of glutor for 24 h (B, C) or with 0.01 µM of glutor for the various indicated time durations (D, E) , followed by estimation of cell viability by Trypan blue dye exclusion assay and cell survival by MTT assay as described in the Materials and Methods section. DL cells incubated in a medium alone or containing glutor (0.01 µM) for 24 h were examined for the mode of cell death by Annexin V/PI (H, I) and Wright–Giemsa (F, G) staining. Values shown in (B–E) and (G, I) are mean ± SD. The plates shown in (A) , (F) , and (H) are from a representative experiment out of at least two experiments with similar results. The bar diagram accompanying (A) is the densitometry of respective bands. * p < 0.05 vs. values of the respective control.
Article Snippet:
Techniques: Transplantation Assay, Western Blot, Incubation, Exclusion Assay, MTT Assay, Staining, Control